The multi‐target mechanism of Cyclosporin A in the treatment of vitiligo based on network pharmacology

Dermatologic TherapyVolume 34, Issue 4 e15023 ORIGINAL ARTICLE The multi-target mechanism of Cyclosporin A in the treatment of vitiligo based on network pharmacology Haojie Lu, Haojie Lu orcid.org/0000-0001-8975-8835 Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorJinhui Xu, Jinhui Xu Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorBo Xie, Bo Xie orcid.org/0000-0001-9374-863X Department of Dermatology, The Third People's Hospital of Hangzhou, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorYebei Hu, Yebei Hu orcid.org/0000-0003-3489-7138 Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorHaixin Luo, Haixin Luo Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorYi Chen, Yi Chen Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorXiuzu Song, Corresponding Author Xiuzu Song songxiuzu@sina.com orcid.org/0000-0001-8272-8131 Department of Dermatology, The Third People's Hospital of Hangzhou, Zhejiang, Hangzhou, China Correspondence Xiuzu Song, Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, Zhejiang, China. Email: songxiuzu@sina.comSearch for more papers by this author Haojie Lu, Haojie Lu orcid.org/0000-0001-8975-8835 Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorJinhui Xu, Jinhui Xu Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorBo Xie, Bo Xie orcid.org/0000-0001-9374-863X Department of Dermatology, The Third People's Hospital of Hangzhou, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorYebei Hu, Yebei Hu orcid.org/0000-0003-3489-7138 Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorHaixin Luo, Haixin Luo Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorYi Chen, Yi Chen Zhejiang Chinese Medical University, Zhejiang, Hangzhou, ChinaSearch for more papers by this authorXiuzu Song, Corresponding Author Xiuzu Song songxiuzu@sina.com orcid.org/0000-0001-8272-8131 Department of Dermatology, The Third People's Hospital of Hangzhou, Zhejiang, Hangzhou, China Correspondence Xiuzu Song, Department of Dermatology, The Third People's Hospital of Hangzhou, Hangzhou, Zhejiang, China. Email: songxiuzu@sina.comSearch for more papers by this author First published: 05 June 2021 https://doi.org/10.1111/dth.15023 Funding information: Basic Public Welfare Research Project of Zhejiang Province, Grant/Award Number: LGF18H110001; National Natural Science Foundation of China, Grant/Award Number: 81872517 Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinked InRedditWechat Abstract Network pharmacology is an emerging discipline that designs drugs based on systems biology theory and biological system network analysis. Here, we applied network pharmacology to analyze the multi-target mechanism of Cyclosporin A in the treatment of vitiligo First, we predicted the targets of Cyclosporin A. Second, we obtained the genes related to vitiligo from the database. Third, we constructed the PPI network of the mutual genes between Cyclosporin A and vitiligo and used gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) to analyze. Finally, we verified the prediction of potential targets through a docking study with Cyclosporin A. We found that there were 15 shared target genes between Cyclosporin A and vitiligo. We analyzed these 15 genes by Cytoscape and obtained a network diagram of 885 nodes. Through screening and molecular docking, PRKDC, CUL7, CUL1, HSPA8, HSPA4, and SIRT7 were the most likely multi-target mechanism of Cyclosporin A in the treatment of vitiligo. In our study, Cyclosporin A might not only affect the repair of DNA strands by targeting PRKDC, but also affected the innate and adaptive immune function of vitiligo patients by the targets of CUL1, CUL7, and HSP70. In addition, Cyclosporin A might promote the repigmentation of vitiligo by adjusting the expression of SIRT7. Open Research DATA AVAILABILITY STATEMENT The datasets used or analyzed during the current study are available from the corresponding author on reasonable request. Supporting Information Filename Description dth15023-sup-0001-TableS1.docWord document, 93.5 KB TABLE S1 The 100 potential targeted proteins dth15023-sup-0002-TableS2.docWord document, 811.4 KB TABLE S2 The 880 related proteins of vitiligo Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume34, Issue4July/August 2021e15023 RelatedInformation