Enhanced Non-Enzymatic Glucose Detection Using a Flower-Like NiCo2O4 Spheres Modified Electrode

The development of non-enzymatic glucose detection method is of great significance in the clinical, biological and food sample analysis. In this work, a NiCo2O4-Nafion modified glassy carbon electrode was successfully fabricated which can rapidly detect glucose (<5 s) with high selectivity. Flower-like nanorod-shaped NiCo2O4 spheres were simply synthesized via an one-pot hydrothermal reaction which were characterized by scanning electron microscope and X-ray diffraction. The as-prepared NiCo2O4 and Nafion modified glassy carbon electrode showed remarkable electrochemical activity for glucose detection in both cyclic voltammetry and chronoamperometry measurements. The anodic current was linearly proportional to glucose concentration in a broad range from 0.1 to 1360 μM. The detection limit of our NiCo2O4−Nafion modified electrode was determined as 10 nM (S/N = 3). Finally, the modified electrode exhibited high stability, selectivity and reproducibility. The electrode has been successfully applied to detect glucose in real food samples.