冷应激
蛋白激酶B
糖原
内分泌学
细胞凋亡
内科学
生物
碳水化合物代谢
体温过低
新陈代谢
医学
生物化学
基因
作者
Yang Liu,Bin Xu,Yajie Hu,Peng Liu,Shuai Lian,Hongming Lv,Yuying Yang,Hong Ji,Huanmin Yang,Juxiong Liu,Ruizhi Yao,Shize Li
出处
期刊:Cryobiology
[Elsevier]
日期:2021-02-27
卷期号:100: 125-132
被引量:19
标识
DOI:10.1016/j.cryobiol.2021.02.008
摘要
Cold stress is one of the serious factors restricting the development of animal husbandry in cold areas. Cold exposure can easily lead to cold stress, slow growth and even death of newborn animals. O-GlcNAcylation modification can act as type of "stress receptor" and"nutrition sensor" in a variety of stress responses, however, it is not clear how O-GlcNAcylation can regulate glucose metabolism in the liver of piglets under cold stress. In this study, piglets 21 days of age were exposed to 4 °C for 4 h or 8 h in a phytotron. Serum cortisol and other stress hormones were used to assess body status to establish a cold stress piglet model. The changes of glycogen in liver were detected by PAS. FDP and PA were also measured to study the glycolysis level of liver. To characterize potential mechanisms of O-GlcNAcylation on the livers of cold stress piglets, AKT, GSK3β, GS, PFKFB2, AS160 and their corresponding phosphorylation were determined by Western blotting. Results show O-GlcNAcylation increased and apoptosis levels increased in the liver following cold exposure during excessive CORT or metabolic dysfunction. It is suggested that the acute cold exposure of piglets induced a sequential change in the level of O-GlcNAcylation, which may be one of the factors mediating liver cell apoptosis and glucose metabolism regulation by the O-GlcNAc/AKT pathway. These findings provide new insight into the mechanisms of the cold stress response, which can facilitate the development of new strategies to combat the effects of hypothermia.
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