The mixed-phase barium titanium oxide perovskite nanoparticles were synthesized by calcining barium carbonate and titanium dioxide precursors at 900 C.The nanoparticles were characterized using X-ray diffraction, Fourier transform infrared spectroscopy, transmission electron microscopy, energy-dispersive X-ray spectroscopy and selective area diffraction analysis.The synthesized barium titanium oxide particles were found to have almost spherical shape with 6-15 nm size.The synthesized mixed-phase BaTiO3/Ba2TiO4 perovskite nanoparticles are used for biofuel production.The formation of methyl ester after the transesterification reaction confirms the conversion of essential oil into biofuel, as analyzed by GC-MS.