Investigation of the transport and absorption of Angelica sinensis polysaccharide through gastrointestinal tract both in vitro and in vivo

当归 体内 体外 胃肠转运 材料科学 胃肠道 多糖 吸收(声学) 药理学 化学 生物化学 医学 生物 生物技术 病理 中医药 复合材料 替代医学 胃肠病学
作者
Kaiping Wang,Fang Cheng,Xianglin Pan,Tao Zhou,Xi‐Qiu Liu,Ziming Zheng,Li Luo,Yu Zhang
出处
期刊:Drug Delivery [Taylor & Francis]
卷期号:24 (1): 1360-1371 被引量:70
标识
DOI:10.1080/10717544.2017.1375576
摘要

To investigate the absorption and delivery of ASP in gastrointestinal (GI) tract, cASP was successfully synthesized by chemically modifying with succinic anhydride and then conjugating with a near infrared fluorescent dye Cy5.5. Then, the capacity of oral absorption of cASP was evaluated. The results demonstrated that cASP had low toxicity and no disruption on the integrity of cell membrane. The endocytosis of cASP into the epithelial cells was time- and energy-dependent, which was mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes. Otherwise, the actin filaments played a relatively weak role at the same time. The transport study illustrated that cASP could penetrate through the epithelial monolayer and mainly mediated by the same routes as that in the endocytosis experiment. Moreover, both in vitro Ussing chamber and in vivo ligated intestinal loops models indicated that cASP could be diffused through the mucus barriers and be absorbed in the whole small intestine. Finally, near-infrared fluorescence imaging presented that cASP could be absorbed and circulated into the blood, then distributed into various organs after oral administration. In conclusion, ASP could be absorbed after oral administration through endocytosis process mainly mediated by macropinocytosis pathway and clathrin- and caveolae (or lipid raft)-related routes, then be absorbed and circulated into blood. This study presents a comprehensive understanding of oral delivery of cASP, which will provide theoretical basis for the clinical application of ASP.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
温暖元柏完成签到,获得积分10
刚刚
芝士大王完成签到 ,获得积分10
刚刚
快乐的秋翠完成签到,获得积分10
刚刚
Zhao完成签到,获得积分10
1秒前
王木木完成签到,获得积分10
1秒前
我来电了完成签到,获得积分10
2秒前
2秒前
XLL小绿绿完成签到,获得积分10
2秒前
夹心就是嘉欣呀完成签到,获得积分10
2秒前
无名花生完成签到 ,获得积分10
3秒前
fei菲飞完成签到,获得积分10
4秒前
缓慢的冬云完成签到,获得积分10
4秒前
来自三百发布了新的文献求助10
4秒前
momo发布了新的文献求助10
5秒前
Haibara5关注了科研通微信公众号
5秒前
biubiu完成签到,获得积分10
6秒前
bjglp完成签到,获得积分10
6秒前
微风完成签到,获得积分10
6秒前
三只眼小怪兽完成签到,获得积分10
7秒前
AJ完成签到 ,获得积分10
9秒前
蒲公英完成签到 ,获得积分10
9秒前
勤劳绿毛龟完成签到,获得积分10
9秒前
雷欧奥特曼完成签到,获得积分10
9秒前
hq完成签到,获得积分10
9秒前
gao完成签到 ,获得积分0
9秒前
hunajx完成签到,获得积分10
9秒前
开心的若烟完成签到,获得积分10
10秒前
来自三百发布了新的文献求助10
10秒前
研友_LMpo68完成签到 ,获得积分10
10秒前
好的昂完成签到,获得积分10
11秒前
Ygy完成签到,获得积分10
11秒前
fann完成签到 ,获得积分10
11秒前
梨梨完成签到,获得积分10
12秒前
auraLyV完成签到,获得积分10
13秒前
科研通AI5应助子车谷波采纳,获得10
14秒前
鹏N完成签到,获得积分10
15秒前
文心同学完成签到,获得积分0
15秒前
zp6666tql完成签到 ,获得积分10
15秒前
断水断粮的科研民工完成签到,获得积分10
15秒前
ff0110完成签到,获得积分10
15秒前
高分求助中
A new approach to the extrapolation of accelerated life test data 1000
Cognitive Neuroscience: The Biology of the Mind 1000
Technical Brochure TB 814: LPIT applications in HV gas insulated switchgear 1000
Immigrant Incorporation in East Asian Democracies 600
Nucleophilic substitution in azasydnone-modified dinitroanisoles 500
不知道标题是什么 500
A Preliminary Study on Correlation Between Independent Components of Facial Thermal Images and Subjective Assessment of Chronic Stress 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 3968603
求助须知:如何正确求助?哪些是违规求助? 3513420
关于积分的说明 11168029
捐赠科研通 3248900
什么是DOI,文献DOI怎么找? 1794540
邀请新用户注册赠送积分活动 875187
科研通“疑难数据库(出版商)”最低求助积分说明 804676