转录组
生物
免疫系统
鹅
基因
硒蛋白
硒
基因表达谱
基因表达
细胞生物学
遗传学
酶
生物化学
超氧化物歧化酶
谷胱甘肽过氧化物酶
化学
古生物学
有机化学
作者
Nan Cao,Wanyan Li,Bingxin Li,Yunbo Tian,Wanyan Li
摘要
Abstract The goose is an economically important poultry species and a principal natural host of avian viruses. This study aimed to determine the effects of selenium on the immune response of geese. Under selenium stimulation, gene expression profiling was investigated using transcriptome sequencing. The selenoproteins were promoted by selenium stimulation, while the heat shock proteins, interleukin and interferons were mainly down‐regulated. After comparison, 2228 differentially expressed genes were primarily involved in immune and environmental response, and infectious disease and genetic information processing related pathways were identified. Specifically, the enzymes of the lysosomes which acted as a safeguard in preventing pathogens were mostly up‐regulated and six randomly selected differentially expressed genes were validated by quantitative polymerase chain reaction. In addition, the most proportional increased transcription factor family basic helix‐loop‐helix ( bHLH ) located in the 5′ flank of selenoprotein P‐like protein for selenium metabolism was identified by response to the selenium stimulation in this study. These analyses show that selenium can promote immune function by activating selenoproteins, transcript factors and lysosome pathway related genes, while weakening cytokine content genes in geese.
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