生物
效应器
RAC1
沙门氏菌
细胞生物学
微生物学
单核细胞增生李斯特菌
膜皱折
细胞骨架
信号转导
细菌
细胞
遗传学
作者
Kyoko E. Yuki,Hadir Marei,Evgenij Fiškin,Megan M. Eva,Angelica A. Gopal,Jeremy Schwartzentruber,Jacek Majewski,Mathieu Cellier,Judith N. Mandl,Silvia M. Vidal,Danielle Malo,Ivan Ðikić
出处
期刊:Nature microbiology
日期:2019-07-08
卷期号:4 (9): 1516-1531
被引量:42
标识
DOI:10.1038/s41564-019-0484-8
摘要
Salmonella presents a global public health concern. Central to Salmonella pathogenicity is an ability to subvert host defences through strategically targeting host proteins implicated in restricting infection. Therefore, to gain insight into the host–pathogen interactions governing Salmonella infection, we performed an in vivo genome-wide mutagenesis screen to uncover key host defence proteins. This revealed an uncharacterized role of CYRI (FAM49B) in conferring host resistance to Salmonella infection. We show that CYRI binds to the small GTPase RAC1 through a conserved domain present in CYFIP proteins, which are known RAC1 effectors that stimulate actin polymerization. However, unlike CYFIP proteins, CYRI negatively regulates RAC1 signalling, thereby attenuating processes such as macropinocytosis, phagocytosis and cell migration. This enables CYRI to counteract Salmonella at various stages of infection, including bacterial entry into non-phagocytic and phagocytic cells as well as phagocyte-mediated bacterial dissemination. Intriguingly, to dampen its effects, the bacterial effector SopE, a RAC1 activator, selectively targets CYRI following infection. Together, this outlines an intricate host–pathogen signalling interplay that is crucial for determining bacterial fate. Notably, our study also outlines a role for CYRI in restricting infection mediated by Mycobacterium tuberculosis and Listeria monocytogenes. This provides evidence implicating CYRI cellular functions in host defence beyond Salmonella infection. A mouse mutagenesis screen identifies CYRI (FAM49B) as a host protection factor against Salmonella infection. CYRI negatively regulates RAC1 signalling to interfere with bacterial entry and dissemination.
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