亲爱的研友该休息了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!身体可是革命的本钱,早点休息,好梦!

Rutin alleviates hypoxia/reoxygenation-induced injury in myocardial cells by up-regulating SIRT1 expression

芦丁 乳酸脱氢酶 丙二醛 药理学 化学 活力测定 超氧化物歧化酶 氧化应激 谷胱甘肽过氧化物酶 细胞凋亡 过氧化氢酶 生物化学 医学 抗氧化剂
作者
Yang Han,Chao Wang,Lingyan Zhang,Jun Lv,Hongzao Ni
出处
期刊:Chemico-Biological Interactions [Elsevier]
卷期号:297: 44-49 被引量:38
标识
DOI:10.1016/j.cbi.2018.10.016
摘要

Rutin possesses multiple pharmacological activities, including the cardioprotective effect. The present study aimed to evaluate the protective effects of rutin on hypoxia/reoxygenation (H/R)-induced myocardial injury and its underlying mechanism involved. H9c2 cells were pretreated with 50 μM rutin or combined with 1 μM silent information regulator 1 (SIRT1) inhibitor (EX-527) for 1 h, and subjected to hypoxia for 6 h, followed by reoxygenation for 24 h. SIRT1 expression was detected by qRT-PCR and western blot. The effects of rutin or combined with EX-527 on cell viability, myocardial injury, apoptotic rate, and oxidative stress in H/R-stimulated H9c2 cells were assayed. The results showed that rutin elevated SIRT1 expression in H9c2 cells, as well as H/R-stimulated H9c2 cells. Rutin increased cell viability in H9c2 cells exposed to H/R. H/R stimulation induced myocardial injury, as evidenced by the increased levels of lactate dehydrogenase (LDH), creatine kinase-MB (CK-MB) and aspartate transaminase (AST), which were abolished in the presence of rutin. Rutin attenuated H/R-induced increase of apoptotic rate and caspase-3 activity in H/R-treated cells. Moreover, H/R-induced decrease in the activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px) and catalase (CAT), and increase in malondialdehyde (MDA) content were reversed by rutin treatment. The presence of EX-527 abolished these protective effects of rutin. In conclusion, rutin protected H9c2 cells against H/R injury through increasing SIRT1 expression. Our findings suggested that rutin might be a potential therapeutic agent for the treatment of myocardial H/R injury.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
13秒前
PP完成签到 ,获得积分20
32秒前
FashionBoy应助mashibeo采纳,获得10
45秒前
1分钟前
1分钟前
FMHChan完成签到,获得积分10
1分钟前
1分钟前
小红书求接接接接一篇完成签到,获得积分20
1分钟前
1分钟前
Ava应助科研通管家采纳,获得20
1分钟前
1分钟前
PP发布了新的文献求助10
2分钟前
舒心豪英完成签到 ,获得积分10
3分钟前
3分钟前
3分钟前
健康幸福平安完成签到,获得积分10
3分钟前
震动的听枫完成签到,获得积分10
3分钟前
mashibeo发布了新的文献求助10
3分钟前
传奇3应助hautzhl采纳,获得10
5分钟前
吴可之完成签到,获得积分10
5分钟前
Langsam发布了新的文献求助10
5分钟前
tutu完成签到,获得积分10
5分钟前
mashibeo完成签到,获得积分10
5分钟前
5分钟前
CipherSage应助科研通管家采纳,获得10
5分钟前
华仔应助科研通管家采纳,获得10
5分钟前
5分钟前
6分钟前
6分钟前
6分钟前
6分钟前
6分钟前
传奇3应助飞常爱你哦采纳,获得10
7分钟前
7分钟前
嗯哼举报生生求助涉嫌违规
7分钟前
7分钟前
7分钟前
jyy应助科研通管家采纳,获得10
7分钟前
7分钟前
8分钟前
高分求助中
좌파는 어떻게 좌파가 됐나:한국 급진노동운동의 형성과 궤적 2500
Sustainability in Tides Chemistry 1500
TM 5-855-1(Fundamentals of protective design for conventional weapons) 1000
Cognitive linguistics critical concepts in linguistics 800
Threaded Harmony: A Sustainable Approach to Fashion 799
Livre et militantisme : La Cité éditeur 1958-1967 500
氟盐冷却高温堆非能动余热排出性能及安全分析研究 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3052513
求助须知:如何正确求助?哪些是违规求助? 2709785
关于积分的说明 7418180
捐赠科研通 2354355
什么是DOI,文献DOI怎么找? 1245876
科研通“疑难数据库(出版商)”最低求助积分说明 605927
版权声明 595908