化学
环介导等温扩增
核酸扩增试验
核酸
计算生物学
DNA
生物化学
生物
免疫学
沙眼衣原体
作者
Cheng Qian,Rui Wang,Hui Wu,Feng Ji,Jian Wu
标识
DOI:10.1016/j.aca.2018.10.054
摘要
Nucleic acid isothermal amplification detection technologies have undergone rapid development in the field of molecular diagnosis due to their freedom from the limitations of complex temperature control equipment. In recent years, with the use of newly discovered nicking endonucleases, a novel isothermal amplification technique, -nicking enzyme-assisted amplification (NEAA), has been developed. NEAA reactions offer high levels of amplification efficiency and can exponentially amplify a target nucleic acid over a very short period of time. NEAA has been widely used in many fields, including those of molecular diagnosis, disease detection, biochemical identification, and many more. NEAA reactions also present some drawbacks, (e.g., severe non-specific amplification and strong background signals.) The principle, advantages and disadvantages of NEAA technologies are discussed. Applications and comparisons of NEAA to similar methods are described in this review. We hope that this review will serve as inspiration for further NEAA research.
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