罗伊乳杆菌
医学
安慰剂
FOXP3型
益生菌
TLR2型
生物
哭
TLR4型
免疫系统
胃肠病学
内科学
受体
免疫学
细菌
病理
精神科
替代医学
遗传学
作者
Francesco Savino,Ilaria Galliano,María Garro,Andrea Savino,Valentina Daprà,Paola Montanari,Massimiliano Bergallo
出处
期刊:Beneficial Microbes
[Wageningen Academic Publishers]
日期:2018-12-07
卷期号:9 (6): 917-925
被引量:21
摘要
Regulatory T cells induce immune homeostasis and the expression of Toll like receptors (TLRs); subsequent inflammatory cytokine release may be involved. Recent studies have shown a microbial imbalance in the gut of colicky infants (with a prevalence of gram-negative bacteria, such as Escherichia coli ), and accumulating evidence has shown the efficacy of a probiotic ( Lactobacillus reuteri ) in breastfed subjects, but the underlying mechanism remains undefined. The study enrolled 59 infants younger than 60 days, of whom 34 subjects had colic and 25 were healthy controls. With a double-blind, placebo-controlled randomised study performed in our unit from October 2016 to July 2017, infants with colic were randomly assigned to receive oral daily L. reuteri DSM17938 (1×10 8 cfu) or placebo for 28 days. Peripheral blood was collected to assess the expression of FoxP3, TLR2 and TLR4 mRNA using real-time TaqMan RT-PCR at baseline and after the study period. Our findings showed increased mRNA expression of the transcription factor forkhead box P3 (FoxP3) in infants treated with L. reuteri DSM 17938 for 28 days ( P <0.009) and increased TLR2 and TLR4 mRNA expression in both treated and placebo subjects. After L. reuteri administration for 28 days in infants with colic, we observed a significant decrease in daily crying time (302.3±19.86 min/day on day 0 vs 76.75±22.15 min/day on day 28, P =0.001). This study provides evidence that the observed increase in FoxP3 expression and reduction in crying time might be responses to probiotic treatment, while the increase in TLR2 and TLR4 mRNA expression might be related to age. Exploiting these new findings may lead to an unprecedented level of therapeutic control over immune tolerance using probiotics.
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