衰老
活性氧
细胞内
细胞生物学
细胞外
生物
线粒体
老化
胚胎干细胞
生物化学
遗传学
基因
作者
Stella Victorelli,João F. Passos
出处
期刊:Methods in molecular biology
日期:2018-11-25
卷期号:: 21-29
被引量:38
标识
DOI:10.1007/978-1-4939-8931-7_3
摘要
Cumulative evidence suggests that cellular senescence plays a variety of important physiological roles, including tumor suppression, embryonic development and ageing. Senescent cells are characterized by increased production of reactive oxygen species (ROS), mostly produced by dysfunctional mitochondria. Both intracellular and extracellular ROS have been shown to contribute to the induction of senescence. ROS have also been shown to act as signaling molecules during senescence, stabilizing the cell-cycle arrest. In this chapter, we present a detailed description of protocols that allow us to characterize intracellular and extracellular ROS in live senescent cells.
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