诱导多能干细胞
生物
胚胎干细胞
细胞生物学
定向微分
细胞分化
多巴胺能
干细胞
电池类型
人诱导多能干细胞
细胞
神经科学
多巴胺
遗传学
基因
作者
Leah Boyer,Benjamin C. Campbell,Samantha Larkin,Yan-Ling Mu,Fred H. Gage
标识
DOI:10.1002/9780470151808.sc01h06s22
摘要
Abstract Here we describe protocols for the dopaminergic differentiation of pluripotent stem cells. We have optimized and compared two distinct protocols, both of which are chemically defined and applicable to both embryonic and induced pluripotent stem cells. First, we present a five‐step method based on rosette formation (Basic Protocol 1); then we describe a monolayer paradigm based on inhibition of alternate developmental pathways (Basic Protocol 2). Directed differentiation of pluripotent cells into specific cell types is a crucial step towards understanding human development and realizing the biomedical relevance of these cells, whether for replacement therapy or disease modeling. Curr. Protoc. Stem Cell Biol . 22:1H.6.1‐1H.6.11. © 2012 by John Wiley & Sons, Inc.
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