去酰胺
合理设计
重组DNA
糖基化
中国仓鼠卵巢细胞
蛋白质工程
生物化学
细胞培养
计算生物学
蛋白质质量
功能(生物学)
生物
细胞生物学
酶
遗传学
基因
作者
David Brühlmann,Martin Jordan,Jürgen Hemberger,Markus Sauer,Matthieu Stettler,Hervé Broly
摘要
Clinical efficacy and safety of recombinant proteins are closely associated with their structural characteristics. The major quality attributes comprise glycosylation, charge variants (oxidation, deamidation, and C- & N-terminal modifications), aggregates, low-molecular-weight species (LMW), and misincorporation of amino acids in the protein backbone. Cell culture media design has a great potential to modulate these quality attributes due to the vital role of medium in mammalian cell culture. The purpose of this review is to provide an overview of the way both classical cell culture medium components and novel supplements affect the quality attributes of recombinant therapeutic proteins expressed in mammalian hosts, allowing rational and high-throughput optimization of mammalian cell culture media. A selection of specific and/or potent inhibitors and activators of oligosaccharide processing as well as components affecting multiple quality attributes are presented. Extensive research efforts in this field show the feasibility of quality engineering through media design, allowing to significantly modulate the protein function.
科研通智能强力驱动
Strongly Powered by AbleSci AI