Effects of Medium Components and Fermentation Conditions on Cytidine Production by Recombinant Escherichia coli CYT20

Recently, cytidine is gaining more attention as a precursor for antiviral drugs. Cytidine is produced by some microorganisms fermentation. Cytidine producing strain Escherichia coli CYT20 was bred in our previous study. Glucose, (NH4)2SO4, and temperature were selected as variables for optimization of fermentation medium and conditions for cytidine production. Among ingredients, different concentrations of initial glucose and (NH4)2SO4 had positive effects on cytidine production, and pH 7.0 and temperature 36 °C were favoured fermentation conditions for cytidine production by E. coli CYT20. After shake flask batch fermentation, production of cytidine was measured by high performance liquid chromatography. The results showed that it was 1,866 mg/l under optimized condition and it was 1,115 mg/l under un-optimized condition, cytidine production was increased by 67.4 %.