罗丹明
显微镜
苯
罗丹明B
化学
风暴
分辨率(逻辑)
光化学
材料科学
纳米技术
光学
荧光
气象学
催化作用
物理
有机化学
计算机科学
人工智能
光催化
作者
Bowen Wang,Michael Xiong,Josephine Susanto,Xue Li,Wai‐Yee Leung,Ke Xu
标识
DOI:10.1002/anie.202113612
摘要
We introduce a strategy to optimize the photoswitching behavior of rhodamines for (d)STORM super-resolution microscopy. By replacing the benzene ring in the rhodamine core with a permanently charged 1,3-disubstituted imidazolium, the resultant dyes are markedly sensitized toward photoswitching, and exhibit outstanding (d)STORM performance with fast on-off switching, long-lasting blinking, and bright single-molecule emission. We thus attain excellent (d)STORM images under green excitation that are on par with the "ideal" red-excited dyes, including for difficult structures such as the mammalian actin cytoskeleton, and demonstrate high-quality two-color three-dimensional (d)STORM.
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