骨桥蛋白
骨整合
成骨细胞
细胞生物学
间质细胞
破骨细胞
骨钙素
医学
病理
植入
化学
生物
免疫学
内科学
碱性磷酸酶
体外
生物化学
酶
受体
外科
作者
Barbara D. Boyan,Michael B. Berger,Fred R. Nelson,Henry J. Donahue,Zvi Schwartz
出处
期刊:Journal of the American Academy of Orthopaedic Surgeons
[American Academy of Orthopaedic Surgeons]
日期:2022-04-05
卷期号:30 (13): e894-e898
标识
DOI:10.5435/jaaos-d-21-00523
摘要
Bone marrow stromal cells are regulated by the chemical and physical features of a biomaterial surface. When grown on titanium (Ti) and Ti alloy surfaces, such as titanium-aluminum-vanadium, with specific topographies that mimic the microscale, mesoscale, and nanoscale features of an osteoclast resorption pit, they undergo a rapid change in cell shape to assume a columnar morphology typical of a secretory osteoblast. These cells exhibit markers associated with an osteoblast phenotype, including osteocalcin and osteopontin, and they secrete factors associated with osteogenesis, including bone morphogenetic protein 2, vascular endothelial growth factor, and neurotrophic semaphorins. The pathway involves a shift in integrin expression from α5β1 to α2β1 and signaling by Wnt5a rather than Wnt3a. Conditioned media from these cultures can stimulate vasculogenesis by human endothelial cells and osteoblastic differentiation of marrow stromal cells not grown on the biomimetic substrate, suggesting that the surface could promote osteogenesis in vivo through similar mechanisms. In vivo studies using a variety of animal models confirm that implants with biomimetic surfaces result in improved osseointegration compared with Ti implants with smooth surfaces, as do meta-analyses comparing clinical performance of implant surface topographies.
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