Intermittent Changes in Temperature and Humidity Repress Gray Mold in Tomato

Environmental temperature and humidity play a vital role in plant-pathogen interactions, which profoundly affect the occurrence of crop diseases. However, the specific methods and mechanisms through which intermittent changes in temperature and humidity mitigate plant diseases remain unclear. In this study, six temperature and humidity combinations were set, the disease severity of tomatoes and biomass of Botrytis cinerea were analyzed, and the infection process of pathogens was observed using an optical microscope. Furthermore, dual RNA-seq analysis was performed to explore the interactions between plants and pathogens. Results showed that the 24 hours postinoculation (hpi)-12 h day (regulation was performed at 24 hpi for 12 h after inoculation during the day) treatment reduced the gray mold severity and biomass of B. cinerea in plants by the greatest amount and effectively inhibited the growth of mycelia. The 24 hpi-12 h day treatment induced the upregulation of light reactions, photorespiration, and Calvin cycle-related genes in tomatoes, whereas fungal genes related to the biosynthesis of sesquiterpene botrydial and polyketide botcinic acid were downregulated. Overall, we identified the optimal combination of temperature and humidity changes to inhibit the development of tomato gray mold and preliminarily explored the interactions between tomato and B. cinerea under temperature and humidity changes. This work has practical importance and provides a theoretical basis for the ecological control of plant diseases.