Lentivirus-Mediated RNAi in Skeletal Myogenesis

RNA interference (RNAi) has greatly facilitated investigation of gene functions in vitro as well as in vivo. Recombinant lentivirus is widely used to deliver small hairpin RNA (shRNA) because of its high transduction capacity into diverse cell types and tissues. Here, we describe methods of lentivirus-mediated delivery of shRNA for the study of skeletal muscle cell differentiation in vitro and injury-induced muscle regeneration in mice.