A collagen-coated sponge silk scaffold for functional meniscus regeneration

Journal of Tissue Engineering and Regenerative MedicineVolume 13, Issue 2 p. 156-173 RESEARCH ARTICLE A collagen-coated sponge silk scaffold for functional meniscus regeneration Ruijian Yan, Ruijian Yan Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaThese co-authors contributed equally to the workSearch for more papers by this authorYangwu Chen, Yangwu Chen Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorYanjia Gu, Yanjia Gu Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorChenqi Tang, Chenqi Tang Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorJiayun Huang, Jiayun Huang Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorYejun Hu, Yejun Hu Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorZefeng Zheng, Zefeng Zheng Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Department of Orthopedic Surgery, The Children's Hospital, School of Medicine, Zhejiang University, Zhejiang, ChinaSearch for more papers by this authorJisheng Ran, Jisheng Ran Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorBoonchin Heng, Boonchin Heng Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong KongSearch for more papers by this authorXiao Chen, Xiao Chen Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, ChinaSearch for more papers by this authorZi Yin, Zi Yin Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, ChinaSearch for more papers by this authorWeishan Chen, Corresponding Author Weishan Chen chenweishan@zju.edu.cn Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Correspondence Weiliang Shen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: wlshen@zju.edu.cn; shenweiliang365@163.com Weishan Chen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: chenweishan@zju.edu.cnSearch for more papers by this authorWeiliang Shen, Corresponding Author Weiliang Shen wlshen@zju.edu.cn orcid.org/0000-0001-8131-325X Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, China Correspondence Weiliang Shen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: wlshen@zju.edu.cn; shenweiliang365@163.com Weishan Chen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: chenweishan@zju.edu.cnSearch for more papers by this authorHongwei Ouyang, Hongwei Ouyang Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, ChinaSearch for more papers by this author Ruijian Yan, Ruijian Yan Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaThese co-authors contributed equally to the workSearch for more papers by this authorYangwu Chen, Yangwu Chen Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorYanjia Gu, Yanjia Gu Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorChenqi Tang, Chenqi Tang Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorJiayun Huang, Jiayun Huang Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorYejun Hu, Yejun Hu Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorZefeng Zheng, Zefeng Zheng Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Department of Orthopedic Surgery, The Children's Hospital, School of Medicine, Zhejiang University, Zhejiang, ChinaSearch for more papers by this authorJisheng Ran, Jisheng Ran Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, ChinaSearch for more papers by this authorBoonchin Heng, Boonchin Heng Faculty of Dentistry, The University of Hong Kong, Pokfulam, Hong KongSearch for more papers by this authorXiao Chen, Xiao Chen Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, ChinaSearch for more papers by this authorZi Yin, Zi Yin Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, ChinaSearch for more papers by this authorWeishan Chen, Corresponding Author Weishan Chen chenweishan@zju.edu.cn Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Correspondence Weiliang Shen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: wlshen@zju.edu.cn; shenweiliang365@163.com Weishan Chen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: chenweishan@zju.edu.cnSearch for more papers by this authorWeiliang Shen, Corresponding Author Weiliang Shen wlshen@zju.edu.cn orcid.org/0000-0001-8131-325X Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, China Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Orthopaedics Research Institute, Zhejiang Univerisity, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, China Correspondence Weiliang Shen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: wlshen@zju.edu.cn; shenweiliang365@163.com Weishan Chen, Department of Orthopedic Surgery, 2nd Affiliated Hospital, School of Medicine, Zhejiang University, 88 Jie Fang Road, Hangzhou 310009, China. Email: chenweishan@zju.edu.cnSearch for more papers by this authorHongwei Ouyang, Hongwei Ouyang Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cell and Regenerative Medicine, Zhejiang University, Hangzhou, China Department of Sports Medicine, School of Medicine, Zhejiang University, Zhejiang, China China Orthopaedic Regenerative Medicine (CORMed), Zhejiang University, Hangzhou, ChinaSearch for more papers by this author First published: 28 November 2018 https://doi.org/10.1002/term.2777Citations: 22 The authors declare there are no financial conflicts of interest. Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Abstract Tissue engineering is a promising solution for meniscal regeneration after meniscectomy. However, in situ reconstruction still poses a formidable challenge due to multifunctional roles of the meniscus in the knee. In this study, we fabricate a silk sponge from 9% (w/v) silk fibroin solution through freeze drying and then coat its internal space and external surface with collagen sponge. Subsequently, various characteristics of the silk-collagen scaffold are evaluated, and cytocompatibility of the construct is assessed in vitro and subcutaneously. The efficacy of this composite scaffold for meniscal regeneration is evaluated through meniscus reconstruction in a rabbit meniscectomy model. It is found that the internally coated collagen sponge enhances the cytocompatibility of the silk sponge, and the external layer of collagen sponge significantly improves the initial frictional property. Additionally, the silk-collagen composite group shows more tissue ingrowth and less cartilage wear than the pure silk sponge group at 3 months postimplantation in situ. These findings thus demonstrate that the composite scaffold had less damage to the joint surface than the silk alone through promoting functional meniscal regeneration after meniscectomy, which indicates its clinical potential in meniscus reconstruction. Citing Literature Supporting Information Filename Description term2777-sup-0001-Supplement_figures_and_table_(revison).docxWord 2007 document , 58.4 KB Figure S1. The Young's modulus of the scaffold. Figure S2. The compressive modulus of the normal meniscus Table S1. Histology evaluation scores of the scaffold. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article. Volume13, Issue2February 2019Pages 156-173 RelatedInformation