TPF imaging of Rhodamine B at different detection windows

Broadband two-photon fluorescence (TPF) imaging system based on femtosecond oscillator is built. Broadband TPF signal generated from Rhodamine B molecule is detected at different detection windows. Both TPF signals detected at short wavelength detection window of 545–615 nm and long wavelength detection window of 642–708 nm are used for TPF imaging. Through switching detection window from short wavelength to long wavelength, deepened imaging depth is achieved. The TPF imaging results, with single [Formula: see text] pixels frame taking time of 4–8 s, demonstrate high performance and the good reliability of the imaging system.