Probiotic lactobacilli mediated changes in global epigenetic signatures of human intestinal epithelial cells during Escherichia coli challenge

益生菌 发酵乳杆菌 鼠李糖乳杆菌 大肠杆菌 生物 表观遗传学 微生物学 DNA甲基化 乙酰化 乳酸菌 碳酸钙-2 细菌 基因表达 遗传学 体外 基因 乳酸 植物乳杆菌
作者
Mohd Iqbal Bhat,Ankita Kumari,Suman Kapila,Rajeev Kapila
出处
期刊:Annals of Microbiology [BioMed Central]
卷期号:69 (6): 603-612 被引量:23
标识
DOI:10.1007/s13213-019-01451-0
摘要

Host genome environment association is critical for proper development and functioning of an individual. Microbiota and probiotics within the cellular vicinity may serve such critical stimuli that can bring out different epigenetic mediated host responses. The aim of present study was to explore the changes in epigenetic signatures of Caco-2 cells by probiotic strains (Lactobacillus rhamnosus MTCC 5897: LR, Lactobacillus fermentum MTCC 5898: LF and their mixture: RF), respectively, or during challenge with Escherichia coli (ATCC 14948) using exclusion, competition and displacement assays. Adenocarcinoma intestinal epithelial Caco-2 cells were treated with LR, LF, RF and E. coli for 6 h, respectively. Caco-2 cells were also challenged with E. coli and probiotic lactobacilli during exclusion, competition and displacement assays. Finally, global epigenetic modifications by acetylation of H4 and H3 histone proteins and DNA methylation patterns were determined. Probiotic-treated Caco-2 cells displayed significant (p < 0.01) reduction in percent global H4 and H3 acetylation, respectively, in contrast to their elevated (p < 0.05) levels after E. coli infection. On the other hand, a remarkable (p < 0.01) decrease in percent H4 and H3 acetylation were observed when E. coli were excluded, competed or displaced by lactobacilli strains. No changes in the global DNA methylation patterns were observed in Caco-2 cells after exposure to probiotic strains or E. coli, respectively, but surprisingly, their levels increased significantly (p < 0.05) when lactobacilli-treated cells were challenged with E. coli during exclusion or competition than displacement assays. Probiotic L. rhamnosus and L. fermentum modulated the host epigenetic signatures via global histone acetylation individually or during E. coli challenge by exclusion, competition and displacement assays. Whilst on the other hand changes in global DNA methylation patterns were obtained significantly during probiotic treatment with E. coli in exclusion and competition protocols.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
PDF的下载单位、IP信息已删除 (2025-6-4)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
潮鸣完成签到 ,获得积分10
刚刚
estrella完成签到 ,获得积分10
2秒前
FYm完成签到,获得积分10
2秒前
3秒前
3秒前
WJing发布了新的文献求助10
4秒前
量子星尘发布了新的文献求助10
4秒前
5秒前
butterfly完成签到,获得积分10
6秒前
丹青完成签到 ,获得积分10
6秒前
6秒前
老魏完成签到,获得积分10
7秒前
大个应助Ther采纳,获得10
7秒前
天才小能喵完成签到 ,获得积分0
7秒前
HuanChen完成签到,获得积分10
8秒前
苗广山完成签到,获得积分10
8秒前
stop here完成签到,获得积分10
8秒前
9秒前
hhh发布了新的文献求助10
9秒前
11秒前
semiaa完成签到,获得积分10
11秒前
汉堡包应助小猪采纳,获得10
14秒前
甜蜜的荟发布了新的文献求助10
14秒前
芜6完成签到,获得积分10
16秒前
berkelerey12138完成签到,获得积分10
16秒前
刘雪完成签到 ,获得积分10
17秒前
完美梨愁完成签到 ,获得积分10
17秒前
yyy完成签到,获得积分10
17秒前
李健的粉丝团团长应助zzzz采纳,获得10
18秒前
Dudidu完成签到,获得积分10
18秒前
一颗大树完成签到,获得积分10
19秒前
潇洒的浩然完成签到,获得积分10
20秒前
比比谁的速度快应助曾珍采纳,获得50
20秒前
22秒前
NiNi完成签到,获得积分10
23秒前
Ther完成签到,获得积分20
24秒前
傲娇白安完成签到,获得积分10
25秒前
25秒前
甜蜜的荟完成签到,获得积分20
26秒前
婷儿完成签到,获得积分10
26秒前
高分求助中
【提示信息,请勿应助】关于scihub 10000
Les Mantodea de Guyane: Insecta, Polyneoptera [The Mantids of French Guiana] 3000
徐淮辽南地区新元古代叠层石及生物地层 3000
The Mother of All Tableaux: Order, Equivalence, and Geometry in the Large-scale Structure of Optimality Theory 3000
Handbook of Industrial Diamonds.Vol2 1100
Global Eyelash Assessment scale (GEA) 1000
Picture Books with Same-sex Parented Families: Unintentional Censorship 550
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 遗传学 基因 物理化学 催化作用 冶金 细胞生物学 免疫学
热门帖子
关注 科研通微信公众号,转发送积分 4038368
求助须知:如何正确求助?哪些是违规求助? 3576068
关于积分的说明 11374313
捐赠科研通 3305780
什么是DOI,文献DOI怎么找? 1819322
邀请新用户注册赠送积分活动 892672
科研通“疑难数据库(出版商)”最低求助积分说明 815029