微泡
流式细胞术
血小板
转染
小RNA
外体
细胞周期
CD63
细胞生物学
基因表达
细胞
化学
生物
分子生物学
基因
免疫学
生物化学
作者
Seyyede Fatemeh Shams,Mahshid Mohammadipour,Mohammad Reza Deyhim
标识
DOI:10.1016/j.jddst.2023.104644
摘要
Platelet-derived exosomes are the most abundant Nano-particles in the plasma of healthy individuals. They have been wildly used as drugs, vectors, and miRs carriers. The present study was designed to hope its results will be used to treat patients with refractory thrombocytopenia. To follow the study's aims, we examined the capacity of platelet-derived exosomes to carry miR-150 and their influence on the expression of c-myb (the target gene of miR-150) and mitosis induction in the megakaryocytic model of M07-e. In this experimental study, platelets were obtained via the platelet concentrates (PCs) prepared by the platelet-rich plasma (PRP) method. Then the exosomes were isolated from the platelets by the isolation kit. The isolated particles were loaded with miR-150 through the modified CaCl2 method. The efficiency of the transfection process was evaluated via qRT-PCR. The effect of loaded exosomes on the gene expression profile (c-myb) and the cell cycle of the megakaryocytic model (M07-e) was assessed via qRT-PCR and flow cytometry methods. Exosomes successfully mediated miR-150 transfer into the cells. As a result, they down modulated c-myb expression significantly (p-value ≤0.05). It means miRs were successfully transferred and did not lose their function; on the other hand, they could not induce cell cycle transition. The findings of the present study shed light on the development of autologous Nano-carriers, which could be a step in advancing the aims of personalized medicine and reducing the side effects of the exogenous carrier.
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