双分子荧光互补
荧光
荧光蛋白
蛋白质-蛋白质相互作用
互补
绿色荧光蛋白
黄色荧光蛋白
蛋白质片段互补分析
化学
生物物理学
细胞生物学
生物
生物化学
物理
基因
量子力学
表型
作者
Prabu Gnanasekaran,H. R. Pappu
出处
期刊:Methods in molecular biology
日期:2023-01-01
卷期号:: 117-120
标识
DOI:10.1007/978-1-0716-3327-4_11
摘要
Bimolecular fluorescence complementation (BiFC) assay is a method to visualize the protein-protein interaction in living cells. This technique is based on ability of the non-fluorescent fragment of fluorescent protein to form fluorescent complex when they are fused to two interacting proteins. In this chapter, we describe the widely used split yellow fluorescent protein (YFP) system to visualize the protein-protein interaction in plant cells.
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