材料科学
耳蜗
生物医学工程
人工耳蜗植入
微流控
内耳
自愈水凝胶
纳米技术
生物相容性
神经科学
工程类
生物
高分子化学
冶金
作者
Yangnan Hu,Jiayue Xing,Hui Zhang,Xinyi Pang,Y. C. Zhai,Hong Cheng,Dongyu Xu,Menghui Liao,Yanru Qi,Danqi Wu,Bin Zhang,Lin Cheng,Bo Chu,Chen Zhang,Yuanjin Zhao,Renjie Chai
标识
DOI:10.1002/adma.202309002
摘要
Organ-on-chips can highly simulate the complex physiological functions of organs, exhibiting broad application prospects in developmental research, disease simulation, as well as new drug research and development. However, there is still less concern about effectively constructing cochlea-on-chips. Here, a novel cochlear organoids-integrated conductive hydrogel biohybrid system with cochlear implant electroacoustic stimulation (EAS) for cochlea-on-a-chip construction and high-throughput drug screening, is presented. Benefiting from the superior biocompatibility and electrical property of conductive hydrogel, together with cochlear implant EAS, the inner ear progenitor cells can proliferate and spontaneously shape into spheres, finally forming cochlear organoids with good cell viability and structurally mature hair cells. By incorporating these progenitor cells-encapsulated hydrogels into a microfluidic-based cochlea-on-a-chip with culture chambers and a concentration gradient generator, a dynamic and high-throughput evaluation of inner ear disease-related drugs is demonstrated. These results indicate that the proposed cochlea-on-a-chip platform has great application potential in organoid cultivation and deafness drug evaluation.
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