生物
背景(考古学)
第四维度
显微镜
低温电子显微
电子断层摄影术
电子显微镜
计算生物学
数据科学
纳米技术
计算机科学
生物系统
统计物理学
生物物理学
材料科学
时空
扫描透射电子显微镜
物理
光学
量子力学
古生物学
作者
Caitlyn L McCafferty,Sven Klumpe,Rommie E. Amaro,Wanda Kukulski,Lucy Collinson,Benjamin D. Engel
出处
期刊:Cell
[Elsevier]
日期:2024-02-01
卷期号:187 (3): 563-584
被引量:33
标识
DOI:10.1016/j.cell.2024.01.005
摘要
Biology spans a continuum of length and time scales. Individual experimental methods only glimpse discrete pieces of this spectrum but can be combined to construct a more holistic view. In this Review, we detail the latest advancements in volume electron microscopy (vEM) and cryo-electron tomography (cryo-ET), which together can visualize biological complexity across scales from the organization of cells in large tissues to the molecular details inside native cellular environments. In addition, we discuss emerging methodologies for integrating three-dimensional electron microscopy (3DEM) imaging with multimodal data, including fluorescence microscopy, mass spectrometry, single-particle analysis, and AI-based structure prediction. This multifaceted approach fills gaps in the biological continuum, providing functional context, spatial organization, molecular identity, and native interactions. We conclude with a perspective on incorporating diverse data into computational simulations that further bridge and extend length scales while integrating the dimension of time.
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