脊髓损伤
乙二醇
自愈水凝胶
祖细胞
PEG比率
神经干细胞
脊髓
降冰片烯
化学
材料科学
生物相容性
生物材料
生物医学工程
干细胞
纳米技术
细胞生物学
医学
神经科学
生物
聚合
有机化学
高分子化学
财务
经济
聚合物
作者
Thomas J. Tigner,Gabrielle Dampf,Ashley Tucker,Yu-Chi Huang,Vipin Jagrit,Abigail J. Clevenger,Arpita Mohapatra,Shreya Raghavan,Jennifer N. Dulin,Daniel L. Alge
标识
DOI:10.1002/adhm.202303912
摘要
Abstract Spinal cord injury (SCI) is a serious condition with limited treatment options. Neural progenitor cell (NPC) transplantation is a promising treatment option, and the identification of novel biomaterial scaffolds that support NPC engraftment and therapeutic activity is a top research priority. The objective of this study was to evaluate in situ assembled poly (ethylene glycol) (PEG)‐based granular hydrogels for NPC delivery in a murine model of SCI. Microgel precursors were synthesized by using thiol‐norbornene click chemistry to react four‐armed PEG‐amide‐norbornene with enzymatically degradable and cell adhesive peptides. Unreacted norbornene groups were utilized for in situ assembly into scaffolds using a PEG‐di‐tetrazine linker. The granular hydrogel scaffolds exhibited good biocompatibility and did not adversely affect the inflammatory response after SCI. Moreover, when used to deliver NPCs, the granular hydrogel scaffolds supported NPC engraftment, did not adversely affect the immune response to the NPC grafts, and successfully supported graft differentiation toward neuronal or astrocytic lineages as well as axonal extension into the host tissue. Collectively, these data establish PEG‐based granular hydrogel scaffolds as a suitable biomaterial platform for NPC delivery and justify further testing, particularly in the context of more severe SCI. This article is protected by copyright. All rights reserved
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