已入深夜,您辛苦了!由于当前在线用户较少,发布求助请尽量完整的填写文献信息,科研通机器人24小时在线,伴您度过漫漫科研夜!祝你早点完成任务,早点休息,好梦!

Fingerprint analysis of dang-gui-Si-Ni decoction and its anticoagulant activity in vivo-in vitro

汤剂 体内 传统医学 生药学 体外 抗凝剂 医学 药理学 化学 生物 生物活性 外科 生物化学 生物技术
作者
Yun Li,Teng-Teng Ren,Shanshan Liu,Ling Zhang,Hong Yi,Qing Li,Liang‐Mian Chen,Hui‐Min Gao,Li-Hua Yan,Xiaoqian Liu,Zhimin Wang
出处
期刊:Journal of Ethnopharmacology [Elsevier BV]
卷期号:325: 117890-117890 被引量:4
标识
DOI:10.1016/j.jep.2024.117890
摘要

Dang-Gui-Si-Ni (DGSN) decoction is a classic prescription in the clinical practice of traditional Chinese Medicine (TCM). DGSN decoction is often used to relieve symptoms of cold coagulation and blood stasis recorded by Treatise on Febrile Diseases (Shang Han Lun) and treat Raynaud's disease, dysmenorrhea, arthritis, migraine in TCM clinic. Accumulated evidences have suggested that this diseases are related to microcirculation disturbance. However, the anticoagulant activity and underlying mechanisms of DGSN decoction responsible for the therapeutic not well understood. The fingerprint and anticoagulant activity in vivo-in vitro of DGSN decoction were evaluated to strengthen the quality control and activity study of formulas. The chemical components of DGSN decoction were analyzed by HPLC and its fingerprint similarity were evaluated by “Chinese Medicine Chromatographic Fingerprint Similarity Evaluation Software (2012 Edition)". The anticoagulant activity of DGSN decoction was assessed by measuring four coagulation factors (PT, TT, APTT, FIB) in vitro. Zebrafish thrombosis model induced by punatinib was established to evaluate the activity of improving microvascular hemodynamics in vivo. Quantitative real-time polymerase chain reaction (q-PCR) were adopted to compare the changes in the RNA expression levels of coagulation factor II (FII), VII (FVII), IX (FIX) and X (FX) in zebrafish thrombosis model. The fingerprint similarity evaluation method of DGSN decoction was established. The results showed that 18 samples had higher similarity (S1–S18 > 0.878). Pharmacodynamic results showed that DGSN decoction could extend PT, TT and APTT, and reduce FIB content in vitro. Meanwhile, it markedly enhanced the cardiac output and blood flow velocity at low dosage (500 μg mL−1) in vivo. q-PCR data demonstrated that DGSN decoction (500 μg mL−1) could downregulate the RNA expression of FII, FVII, FIX and FX. Interestingly, there were a bidirectional regulation of FII, FIX and FX in a certain concentration range. In general, DGSN decoction can significantly improve hemodynamics and downregulate coagulation factors, and the results were consistent both in vitro - in vivo. The fingerprint study provide a new perspective for improving the quality control of DGSN decoction. DGSN decoction possess anticoagulant activity by regulating multiple coagulation factors simultaneously. Thus, it has the potential to develop into the novel raw material of anticoagulant drugs.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
陶醉的凡桃完成签到,获得积分20
刚刚
1秒前
1秒前
5秒前
6秒前
脑洞疼应助就这样堕落采纳,获得10
6秒前
srs发布了新的文献求助10
6秒前
幸福剑身发布了新的文献求助10
7秒前
不解歌完成签到,获得积分10
7秒前
格子布发布了新的文献求助10
9秒前
量子星尘发布了新的文献求助10
10秒前
11秒前
深情安青应助T510采纳,获得10
11秒前
喵喵发布了新的文献求助10
12秒前
nicheng发布了新的文献求助10
12秒前
13秒前
余姚发布了新的文献求助10
14秒前
123发布了新的文献求助10
20秒前
21秒前
务实道罡完成签到,获得积分10
21秒前
酷波er应助科研通管家采纳,获得10
22秒前
所所应助科研通管家采纳,获得10
22秒前
脑洞疼应助科研通管家采纳,获得10
22秒前
上官若男应助科研通管家采纳,获得10
22秒前
12完成签到,获得积分10
22秒前
科研通AI5应助jiaojiao采纳,获得30
23秒前
量子星尘发布了新的文献求助10
24秒前
赘婿应助123采纳,获得10
24秒前
26秒前
CLMY发布了新的文献求助10
27秒前
Jasper应助优雅沅采纳,获得10
27秒前
不解歌发布了新的文献求助10
27秒前
欢快的芹菜完成签到,获得积分10
28秒前
31秒前
科研通AI5应助srs采纳,获得10
31秒前
33秒前
芋泥发布了新的文献求助10
33秒前
宅宅完成签到 ,获得积分10
34秒前
35秒前
36秒前
高分求助中
Production Logging: Theoretical and Interpretive Elements 2700
Neuromuscular and Electrodiagnostic Medicine Board Review 1000
Statistical Methods for the Social Sciences, Global Edition, 6th edition 600
こんなに痛いのにどうして「なんでもない」と医者にいわれてしまうのでしょうか 510
ALUMINUM STANDARDS AND DATA 500
Walter Gilbert: Selected Works 500
An Annotated Checklist of Dinosaur Species by Continent 500
热门求助领域 (近24小时)
化学 材料科学 医学 生物 工程类 有机化学 物理 生物化学 纳米技术 计算机科学 化学工程 内科学 复合材料 物理化学 电极 遗传学 量子力学 基因 冶金 催化作用
热门帖子
关注 科研通微信公众号,转发送积分 3666176
求助须知:如何正确求助?哪些是违规求助? 3225281
关于积分的说明 9762138
捐赠科研通 2935195
什么是DOI,文献DOI怎么找? 1607492
邀请新用户注册赠送积分活动 759217
科研通“疑难数据库(出版商)”最低求助积分说明 735166