The effect of osteocyte‐derived RANKL on bone graft remodeling: An in vivo experimental study

DMP1型 骨细胞 兰克尔 骨吸收 吸收 组织学 破骨细胞 化学 骨重建 病理 医学 内分泌学 内科学 成骨细胞 激活剂(遗传学) 受体 体外 生物化学 病毒基质蛋白 基因
作者
Balázs Fehér,Carina Kampleitner,Patrick Heimel,Stefan Tangl,Jill A. Helms,Uwe Küchler,Reinhard Gruber
出处
期刊:Clinical Oral Implants Research [Wiley]
卷期号:34 (12): 1417-1427
标识
DOI:10.1111/clr.14187
摘要

Autologous bone is considered the gold standard for grafting, yet it suffers from a tendency to undergo resorption over time. While the exact mechanisms of this resorption remain elusive, osteocytes have been shown to play an important role in stimulating osteoclastic activity through their expression of receptor activator of NF-κB (RANK) ligand (RANKL). The aim of this study was to assess the function of osteocyte-derived RANKL in bone graft remodeling.In Tnfsf11fl/fl ;Dmp1-Cre mice without osteocyte-specific RANKL as well as in Dmp1-Cre control mice, 2.6 mm calvarial bone disks were harvested and transplanted into mice with matching genetic backgrounds either subcutaneously or subperiosteally, creating 4 groups in total. Histology and micro-computed tomography of the grafts and the donor regions were performed 28 days after grafting.Histology revealed marked resorption of subcutaneous control Dmp1-Cre grafts and new bone formation around subperiosteal Dmp1-Cre grafts. In contrast, Tnfsf11fl/fl ;Dmp1-Cre grafts showed effectively neither signs of bone resorption nor formation. Quantitative micro-computed tomography revealed a significant difference in residual graft area between subcutaneous and subperiosteal Dmp1-Cre grafts (p < .01). This difference was not observed between subcutaneous and subperiosteal Tnfsf11fl/fl ;Dmp1-Cre grafts (p = .17). Residual graft volume (p = .08) and thickness (p = .13) did not differ significantly among the groups. Donor area regeneration was comparable between Tnfsf11fl/fl ;Dmp1-Cre and Dmp1-Cre mice and restricted to the defect margins.The results suggest an active function of osteocyte-derived RANKL in bone graft remodeling.
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