Salicylic acid attenuates brassinosteroid signaling via protein de‐S‐acylation

Article22 May 2023 Source Data Salicylic acid attenuates brassinosteroid signaling via protein de-S-acylation Xiaoshi Liu Xiaoshi Liu orcid.org/0000-0002-2851-7330 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation, Writing - original draft Search for more papers by this author Zian Chen Zian Chen Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Liting Huang Liting Huang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Youwei Ouyang Youwei Ouyang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Zhiying Wang Zhiying Wang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Shuang Wu Shuang Wu Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Weixian Ye Weixian Ye Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Boya Yu Boya Yu Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Yihang Zhang Yihang Zhang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Chengwei Yang Corresponding Author Chengwei Yang [email protected] orcid.org/0000-0002-2648-3181 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: Supervision Search for more papers by this author Jianbin Lai Corresponding Author Jianbin Lai [email protected] orcid.org/0000-0002-9269-9052 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: Supervision, Writing - original draft, Writing - review & editing Search for more papers by this author Xiaoshi Liu Xiaoshi Liu orcid.org/0000-0002-2851-7330 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation, Writing - original draft Search for more papers by this author Zian Chen Zian Chen Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Liting Huang Liting Huang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Youwei Ouyang Youwei Ouyang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Zhiying Wang Zhiying Wang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Shuang Wu Shuang Wu Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Weixian Ye Weixian Ye Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Boya Yu Boya Yu Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Yihang Zhang Yihang Zhang Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: ​Investigation Search for more papers by this author Chengwei Yang Corresponding Author Chengwei Yang [email protected] orcid.org/0000-0002-2648-3181 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: Supervision Search for more papers by this author Jianbin Lai Corresponding Author Jianbin Lai [email protected] orcid.org/0000-0002-9269-9052 Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China Contribution: Supervision, Writing - original draft, Writing - review & editing Search for more papers by this author Author Information Xiaoshi Liu1,†, Zian Chen1,†, Liting Huang1,†, Youwei Ouyang1,†, Zhiying Wang1, Shuang Wu1, Weixian Ye1, Boya Yu1, Yihang Zhang1, Chengwei Yang *,1 and Jianbin Lai *,1 1Guangdong Provincial Key Laboratory of Biotechnology for Plant Development, School of Life Science, South China Normal University, Guangzhou, China † These authors contributed equally to this work *Corresponding author. Tel: +86 20 85210855; E-mail: [email protected] *Corresponding author. Tel: +86 20 85210855; E-mail: [email protected] The EMBO Journal (2023)42:e112998https://doi.org/10.15252/embj.2022112998 Full textView the full text of the articlePDFDownload PDF of article text and main figures.PDF PLUSDownload PDF of article text, main figures, expanded view figures and appendix. Peer ReviewDownload a summary of the editorial decision process including editorial decision letters, reviewer comments and author responses to feedback. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions Figures & Info Abstract Brassinosteroids (BRs) are important plant hormones involved in many aspects of development. Here, we show that BRASSINOSTEROID SIGNALING KINASEs (BSKs), key components of the BR pathway, are precisely controlled via de-S-acylation mediated by the defense hormone salicylic acid (SA). Most Arabidopsis BSK members are substrates of S-acylation, a reversible protein lipidation that is essential for their membrane localization and physiological function. We establish that SA interferes with the plasma membrane localization and function of BSKs by decreasing their S-acylation levels, identifying ABAPT11 (ALPHA/BETA HYDROLASE DOMAIN-CONTAINING PROTEIN 17-LIKE ACYL PROTEIN THIOESTERASE 11) as an enzyme whose expression is quickly induced by SA. ABAPT11 de-S-acylates most BSK family members, thus integrating BR and SA signaling for the control of plant development. In summary, we show that BSK-mediated BR signaling is regulated by SA-induced protein de-S-acylation, which improves our understanding of the function of protein modifications in plant hormone cross talk. Synopsis The subcellular localization and function of Arabidopsis BRASSINOSTEROID SIGNALING KINASEs (BSKs) is maintained by their S-acylation. This report describes how salicylic acid attenuates brassinosteroid signaling by inducing the expression of an enzyme which removes this tether. Most members of the Arabidopsis BSK family are S-acylated for their plasma membrane localization in plant cells. S-acylation is necessary for the function of BSKs but SA suppresses their function by decreasing their S-acylation. ABAPT11 is the enzyme that de-S-acylates BSKs, disrupting their plasma membrane localization. SA induces ABAPT11 expression, suppressing BR-mediated development via the de-S-acylation of BSKs. Previous ArticleNext Article Read MoreAbout the coverClose modalView large imageVolume 42,Issue 13,03 July 2023This cover highlights the article NALCN-mediated sodium influx confers metastatic prostate cancer cell invasiveness by Antoine Folcher, Dmitri Gordienko, Oksana Iamshanova, Natalia Prevarskaya and colleagues. Sustained Na+ influx via the sodium leak channel (NALCN) in aggressive prostate cancer cells recruits plasma membrane and mitochondrial Na+/Ca2+ exchangers, Ca2+ pumps and Ca2+-permeable ion channels to generate long-lasting Ca2+ oscillations. These, in turn, condition Src-regulated actin remodeling, Ca2+-dependent secretion of proteolytic enzymes and invadopodia formation, thus increasing cancer cell invasiveness. (Scientific image by Dmitri Gordienko, University of Lille, France) Volume 42Issue 133 July 2023In this issue ReferencesRelatedDetailsLoading ...