Extracellular Z-DNA Enhances Cariogenicity of Biofilm

生物膜 细胞外基质 细胞外 DNA 胞外聚合物 变形链球菌 胞外多糖 化学 微生物学 功能(生物学) 生物 多糖 细胞生物学 细菌 生物化学 遗传学
作者
Sili Han,Jing Wang,Haisheng Wang,Yu Peng,Lu‐Yang Wang,Yangdi Ou,Li Ding,Jumpei Washio,Nobuhiro Takahashi,Lei Zhang
出处
期刊:Journal of Dental Research [SAGE]
标识
DOI:10.1177/00220345251316822
摘要

Extracellular DNA (eDNA) is one of the core components of the extracellular matrix (ECM) in biofilms and provides attachment sites for microbes and other ECM components. However, little is known about the functions and underlying mechanisms of eDNA in the cariogenicity of dental plaque biofilms. A recent study demonstrated that conformational diversity of eDNA exists in biofilms, and the transition of eDNA from right-handed (B-DNA) to left-handed (Z-DNA) is associated with the structural stability and pathogenicity of biofilms. Caries-related biofilm is a complex multispecies microenvironment. The presence and biological function of the conformational transition of eDNA within this biofilm have not been previously reported. In this study, we found that extracellular Z-DNA is widely present in carious tissues and cariogenic biofilm, especially Streptococcus mutans , indicating its possible role in the occurrence and activity of dental caries. The content of extracellular Z-DNA showed species heterogeneity. The modulation of Z-DNA formation affected the level of extracellular polysaccharide. Increased formation of Z-DNA substantially strengthened the cariogenicity of the biofilm by increasing DNase resistance, structural density, and acid production. These insights provide a new perspective to understand the underlying function of the conformation transition of eDNA in promoting carious lesions, as well as a possible anti-biofilm strategy targeting extracellular Z-DNA.

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