生物相容性
骨桥蛋白
材料科学
锶
骨钙素
碱性磷酸酶
运行x2
鱼腥草素骨
纳米技术
生物化学
化学
生物
有机化学
免疫学
酶
冶金
作者
Xiu Jia,Peijun Wang,Sen Mu,Jiaqun Yan,Jiayin Dai,Liangjia Bi
出处
期刊:NANO
[World Scientific]
日期:2022-11-14
卷期号:17 (14)
被引量:2
标识
DOI:10.1142/s1793292022501089
摘要
Strontium-doped hydroxyapatite nanowires with hierarchical interfaces were prepared in this study by a one-step hydrothermal method using Pluronic123 as a structural guide. The cytotoxicity and biocompatibility of the materials were detected by the MTT assay. The adhesion and growth of MG63 (osteosarcoma cells) on materials with different doping ratios were observed by scanning electron microscopy and fluorescence microscopy. Real-time polymerase chain reaction (RT-PCR) was used to detect the transcription levels of alkaline phosphatase (ALP), Runt-associated transcription factor 2 (Runx2), osteopontin (OPN), type I collagen (Coll-I) and osteocalcin (OCN). The protein expression levels of ALP, Runx2, OPN, Col-I and OCN were detected by western blotting (WB). The results showed that strontium-doped hydroxyapatite nanowires had good biocompatibility and osteogenic induction ability, and the strontium-doped hydroxyapatite nanowires with a 1% strontium content exhibited a better osteogenic induction performance. Therefore, it is expected to become a new, promising bone induction scaffold material.
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