作者
Ali H. Shaib,Abed Alrahman Chouaib,Rajdeep Chowdhury,Jonas Altendorf,Daniel B. Mihaylov,Chi Zhang,D L Krah,Vanessa Imani,Russell K. W. Spencer,Svilen Veselinov Georgiev,Nikolaos Mougios,Mehar Monga,Sofiia Reshetniak,Tiago Mimoso,Han Chen,Parisa Fatehbasharzad,Dagmar Crzan,Kim‐Ann Saal,Mohamad Mahdi Alawieh,Nadia Alawar,Janna Eilts,Jinyoung Kang,Alireza Soleimani,Marcus Müller,Constantin Pape,Luis Álvarez,Claudia Trenkwalder,Brit Mollenhauer,Tiago F. Outeiro,Sarah Köster,Julia Preobraschenski,Ute Becherer,Tobias Moser,Edward S. Boyden,A.R. Aricescu,Markus Sauer,Felipe Opazo,Silvio O. Rizzoli
摘要
The attainable resolution of fluorescence microscopy has reached the subnanometer range, but this technique still fails to image the morphology of single proteins or small molecular complexes. Here, we expand the specimens at least tenfold, label them with conventional fluorophores and image them with conventional light microscopes, acquiring videos in which we analyze fluorescence fluctuations. One-step nanoscale expansion (ONE) microscopy enables the visualization of the shapes of individual membrane and soluble proteins, achieving around 1-nm resolution. We show that conformational changes are readily observable, such as those undergone by the ~17-kDa protein calmodulin upon Ca