细胞外小泡
小泡
纳米技术
化学
计算机科学
生物
材料科学
细胞生物学
生物化学
膜
作者
Xinrui Gu,Lei He,Shouxin Zhang,Hongpan Xu,Han Shen,Rongrong Huang,Zhiyang Li
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2024-10-24
标识
DOI:10.1021/acssensors.4c00315
摘要
Extracellular vesicles (EVs) are emerging biomarkers in liquid biopsy that have gained increasing attention in disease diagnosis and prognosis monitoring. Most reported detection methods require the isolation of EVs from complex body liquids, often involving multiple washing steps to remove excess reagents and eliminate background interference. Nonetheless, these methods not only cause the loss of EVs but also result in poor repeatability and prolonged detection duration. The focus on wash-free detection methods is increasing due to the specific ability to avoid the removal of surplus reagents and, in some cases, even the isolation and purification of EVs. Viewing from different methodological perspectives, this review summarizes the recent advances in wash-free detection of EVs, containing aggregation induction, proximity sensing, allosteric probes, phase separation, Roman spectroscopy, field-effect transistor and microcantilever. The pros and cons of each detection strategy are impartially evaluated and this review concludes the prospects for future developments in this field.
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