细菌粘附素
副干酪乳杆菌
突变体
粘附
分拣酶
微生物学
殖民地化
化学
细菌
基因
生物膜
大肠杆菌
生物
生物化学
乳酸
遗传学
有机化学
细菌蛋白
作者
Luyao Xiao,Xiaogan Zhao,Long Lin,Ghahvechi Chaeipeima Mahsa,Kai Ma,Changliang Zhang,Xin Rui,Wei Li
标识
DOI:10.1021/acs.jafc.4c04256
摘要
The intestinal retention and persistence of lactic acid bacteria (LAB) are strain-specific and affected by the bacterial surface components. However, the contribution of surface adhesins of LAB to intestinal adhesion and colonization remains unclear. In the present study, seven gene knockout mutants (genes related to surface adhesin synthesis) of Lacticaseibacillus paracasei S-NB were derived based on the Cre-lox-based recombination system. Results showed that the capsule layer appeared thinner in the cell wall of S-NBΔ7576, S-NBΔdlt, and S-NBΔsrtA mutants when compared with the wild-type (WT) S-NB. The effects of S-NB_7576 (wzd and wze genes, responsible for capsular polysaccharide synthesis) and S-NB_srtA (sortase A) mutation on the hydrophobicity, surface charge, and adhesion ability seem to vary strongly among seven mutant strains. In vivo colonization experiments showed a decrease in the colonization numbers of S-NBΔ7576 and S-NBΔsrtA in both the ileal and colon lumen from 2 to 8 days when compared with those of the WT S-NB. In conclusion, the synthesis of capsular polysaccharides and the transport of surface proteins are closely related to the adhesion ability and intestinal colonization of L. paracasei S-NB.
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