电子显微镜
抗原
恶性疟原虫
细胞骨架
抗体
细胞生物学
细胞
生物
显微镜
化学
免疫学
物理
光学
疟疾
遗传学
作者
Marek Cyrklaff,Cecília P. Sanchez,Lukas Hanebutte,Julia Jäger,Ulrich S. Schwarz,Michael Lanzer
出处
期刊:Methods in molecular biology
日期:2022-01-01
卷期号:: 445-455
标识
DOI:10.1007/978-1-0716-2189-9_33
摘要
Immuno-electron microscopy can detect and localize antigens in cells or tissues at a resolution of several nanometers. In the case of P. falciparum-infected erythrocytes, immuno-EM studies are frequently hampered by the electron-dense nature of the hemoglobin and access of antibodies to antigenic sites, particularly if the targeted protein is presented on the host cell surface or lies in proximity to the host cell cytoskeleton. Here, we describe an improved immuno-EM protocol that overcomes these problems. The improved signal to noise ratio and the enhanced access to antigenic sites now allows one to obtain information regarding target density and distribution and, hence, additional insights into the architecture and function of parasite-induced, or -affected, structures.
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