咪唑安定
色谱法
气相色谱法
电子俘获
等离子体
化学
医学
麻醉
物理
有机化学
核物理学
离子
镇静
作者
Heonjoo Ha,B Funk,P. Maitre,Alex M. Zbinden,Ferenc Folláth,D. Thomson
出处
期刊:Clinical Chemistry
[Oxford University Press]
日期:1988-04-01
卷期号:34 (4): 676-679
被引量:18
标识
DOI:10.1093/clinchem/34.4.676
摘要
The present assay was developed for quantifying midazolam in plasma of patients hospitalized in intensive-care units or undergoing anesthesia and receiving many other drugs as well. Plasma samples are alkalinized with NaOH and midazolam is extracted into n-hexane. The organic phase is evaporated and reconstituted in n-butyl acetate, and the midazolam is quantified by gas-liquid chromatography with electron-capture detection. The calibration graph for midazolam was linear in the ranges 5-200 and 200-800 micrograms/L. The CVs for precision and reproducibility of the assay were less than 8%. The method was very specific for midazolam; most of the drugs commonly used in anesthesia and in the intensive-care unit did not interfere with the assay. The lowest detectable concentration was 1 microgram/L. The method is adaptable for use with an automated chromatographic system.
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