流式细胞术
化学
体内
纳米技术
透射电子显微镜
柠檬酸
生物物理学
材料科学
核化学
生物化学
分子生物学
生物
生物技术
作者
Xiaoming Zhao,Tianyang Qi,Bai Yang,Wenjing Zhang,Chenfei Kong,Miao Huang,Yuqian Wang,Hao Zhang,Bai Yang,Jie Yang,Jinlan Jiang
出处
期刊:Nanomedicine
日期:2020-03-01
卷期号:15 (7): 677-689
被引量:17
标识
DOI:10.2217/nnm-2019-0390
摘要
Aim: Procaine-derived carbon dots, termed P-dots, expectedly offer both fluorescent biomarker function and anticancer activity. Materials & methods: P-dots were synthesized by condensing procaine, citric acid and ethylenediamine via hydrothermal synthesis and characterized by transmission electron microscopy, Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. The cellular uptake behavior and the bioimaging performance of P-dots were assessed by confocal laser scanning microscopy. Their antitumor activity was evaluated using the CCK-8 assays and in vivo antitumor experiments, and the underlying mechanism was evaluated by flow cytometry and western blotting. Results: P-dots exhibited excellent luminescence properties suitable for bioimaging and considerable anticancer activity associated with caspase-3-related cell apoptosis. Conclusion: The synthesized procaine-derived carbon dots presented a dual function consisting of bioimaging and anticancer activity, which may enable their implementation as safe and effective clinical nanotherapeutics.
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