细胞培养
三维细胞培养
顺铂
球体
多西紫杉醇
表阿霉素
生物
三阴性乳腺癌
癌症研究
癌症
乳腺癌
化疗
遗传学
作者
Masako Muguruma,Saeko Teraoka,Kana Miyahara,Atsushi Ueda,Mariko Asaoka,Miki Okazaki,Takahiko Kawate,Masahiko Kuroda,Yohei Miyagi,Takashi Ishikawa
标识
DOI:10.1016/j.bbrc.2020.08.075
摘要
Three-dimensional (3D) culture reflects tumor biology complexities compared with two-dimensional (2D) culture. Thus, 3D culture has attracted attention in cell biology studies including drug sensitivity tests. Herein, we investigated differences in anticancer drug sensitivities between 2D and 3D culture systems in triple-negative breast cancer (TNBC) cell lines. Thirteen TNBC cell lines were maintained in 2D and 3D cultures for 3 days before drug exposure. Cell morphology in the 3D culture was examined by phase-contrast microscopy. Sensitivities to epirubicin (EPI), cisplatin (CDDP), and docetaxel (DTX) were investigated by cell viability assay in both cultures and compared. The IC50s of all 3 drugs were significantly higher in the 3D culture than in the 2D culture in most cell lines. Those were correlated between the 2D and 3D cultures in EPI (R = 0.555) and CDDP (R = 0.955), but not in DTX (R = 0.221). Round spheroid-forming cells were more resistant to agents than grape-like types. In conclusion, 3D culture was more resistant to all 3 drugs than 2D culture in most TNBC cell lines. Sensitivity to CDDP was highly correlated between the 2D and 3D cultures, but not to DTX. 2D culture may be acceptable for sensitivity test for DNA-damaging agents.
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