生物制药
程序性细胞死亡
生物过程
细胞内
细胞生物学
细胞凋亡
细胞生长
细胞
细胞培养
细胞外
生物
生物反应器
生物技术
生物化学
遗传学
植物
古生物学
作者
Anandrao Ashok Patil,Sachin Ashok Bhor,Won Jong Rhee
标识
DOI:10.1016/j.jiec.2020.08.009
摘要
Mammalian cell cultures are widely used in the biopharmaceutical industry to produce monoclonal antibodies, vaccines, growth factors, etc. Cell death is an essential biological process for physiological growth and development, but it is a major problem in biopharmaceutical production in bio-industry. Cell death within bioreactor occurs due to various intracellular and extracellular stresses. These stresses negatively affect the culture longevity, overall product quality, and yield. Among all cell death types, apoptosis accounts for most of the cellular death in the bioreactor. The implementation and development of various strategies to prevent the cellular death are crucial for robust bioprocess development. Cell death during culture can be prevented or inhibited by supplementing media with specific chemicals, synthetic inhibitors, and genetic cell engineering approaches. In this review, we classified and described different types of cell death and their molecular mechanisms and summarized the cell death inhibition approaches implemented to inhibit cell death for various applications.
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