In Situ Fabrication of Ultrasmall Gold Nanoparticles/2D MOFs Hybrid as Nanozyme for Antibacterial Therapy

Abstract As one of the common reactive oxygen species, H 2 O 2 has been widely used for combating pathogenic bacterial infections. However, the high dosage of H 2 O 2 can induce undesired damages to normal tissues and delay wound healing. In this regard, peroxidase‐like nanomaterials serve as promising nanozymes, thanks to their positive promotion toward the antibacterial performance of H 2 O 2 , while avoiding the toxicity caused by the high concentrations of H 2 O 2 . In this work, ultrasmall Au nanoparticles (UsAuNPs) are grown on ultrathin 2D metal–organic frameworks (MOFs) via in situ reduction. The formed UsAuNPs/MOFs hybrid features both the advantages of UsAuNPs and ultrathin 2D MOFs, displaying a remarkable peroxidase‐like activity toward H 2 O 2 decomposition into toxic hydroxyl radicals (·OH). Results show that the as‐prepared UsAuNPs/MOFs nanozyme exhibits excellent antibacterial properties against both Gram‐negative ( Escherichia coli ) and Gram‐positive ( Staphylococcus aureus ) bacteria with the assistance of a low dosage of H 2 O 2 . Animal experiments indicate that this hybrid material can effectively facilitate wound healing with good biocompatibility. This study reveals the promising potential of a hybrid nanozyme for antibacterial therapy and holds great promise for future clinical applications.