共焦显微镜
共焦
显微镜
荧光显微镜
显微镜
材料科学
荧光
光学
物理
标识
DOI:10.1002/0471142727.mb1411s81
摘要
Abstract This unit introduces the reader to the basic principles of confocal microscopy and the design and capabilities of current confocal microscopes. The advantages and disadvantages of confocal microscopy compared to other techniques for fluorescence imaging are described. There are also practical guidelines for sample preparation and optimization of imaging parameters, as well as examples of some of the applications of confocal microscopy. Curr. Protoc. Mol. Biol . 81:14.11.1‐14.11.18. © 2008 by John Wiley & Sons, Inc.
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