Method for Noninvasive Analysis of Proteins and Small Molecules from Ancient Objects

壁画 化学 背景(考古学) 分子 小分子 纳米技术 高分子科学 绘画 考古 色谱法 有机化学 艺术 生物化学 艺术史 材料科学 历史
作者
Marcello Manfredi,E. Barberis,Fabio Gosetti,Eleonora Conte,Giorgio Gatti,Clara Mattu,Elisa Robotti,Gleb Zilberstein,Igor Koman,Svetlana Zilberstein,Emilio Marengo,Pier Giorgio Righetti
出处
期刊:Analytical Chemistry [American Chemical Society]
卷期号:89 (6): 3310-3317 被引量:55
标识
DOI:10.1021/acs.analchem.6b03722
摘要

Proteins and small molecules from ancient objects and cultural heritage can provide key information and contribute to study the context of objects and artists. However, all present-day protocols and strategies for the analysis of ancient samples are often invasive and require microsampling. Here, we present a new method for the noninvasive analysis of proteins and small molecules: the technique uses a special ethyl-vinyl acetate film functionalized with strong cation/anion exchange and C8 resins, for interacting with both proteins and small molecules present on the surface of the objects, followed by LC–MS/MS analysis. The new method was fully validated for the determination of both proteins and small molecules on several types of supports, showing excellent analytical performances such as, for example, R2 of the calibration curve of 0.98 and 0.99 for proteins and small molecules, low but very repeatable recoveries, particularly adequate for investigations on precious ancient samples that must not be altered by the analytical procedure. ESEM images and LED multispectral imaging confirmed that no damages or alterations occurred onto the support surfaces and no residues were left from the extractive film. Finally, the new method was applied for the characterization of the binders of a historical fresco of the XVI century from the Flemish painter Paul Brill and of a recently discovered fresco from Isidoro Bianchi (XVII century). Moreover the method was employed for the identification of the colorant used by Pietro Gallo (XIV century) on a wood panel. The method here reported can be easily applied to any other research on ancient precious objects and cultural heritage, since it does not require microsampling and the proteins/small molecules extraction can be performed directly in situ, leaving the object unchanged and intact.

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
cheer发布了新的文献求助30
2秒前
2秒前
爻解应助研友_V8Qmr8采纳,获得20
2秒前
3秒前
CipherSage应助trh采纳,获得10
3秒前
2386发布了新的文献求助100
3秒前
奕初阳发布了新的文献求助10
3秒前
3秒前
4秒前
大气摩托发布了新的文献求助10
4秒前
嗯嗯嗯完成签到,获得积分10
5秒前
5秒前
啦啦啦完成签到,获得积分10
7秒前
11MZ发布了新的文献求助10
7秒前
科研顺利发布了新的文献求助10
7秒前
茕穹完成签到,获得积分10
8秒前
傲慢葫芦发布了新的文献求助10
8秒前
9秒前
9秒前
保亮完成签到,获得积分10
10秒前
10秒前
10秒前
还单身的语琴完成签到,获得积分10
12秒前
邓佳鑫Alan应助英俊的筝采纳,获得10
13秒前
爆米花应助陶醉大侠采纳,获得30
13秒前
CipherSage应助Allen5546采纳,获得10
14秒前
11MZ完成签到,获得积分10
14秒前
ding应助傲慢葫芦采纳,获得10
14秒前
14秒前
力阿完成签到,获得积分20
15秒前
研友_nEoMy8发布了新的文献求助10
15秒前
高强发布了新的文献求助10
15秒前
这0发布了新的文献求助10
16秒前
16秒前
顺利毕业发布了新的文献求助10
16秒前
17秒前
执着麦片应助失眠的巧荷采纳,获得20
19秒前
1204仙女发布了新的文献求助10
19秒前
rylynn发布了新的文献求助10
21秒前
22秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 830
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3248411
求助须知:如何正确求助?哪些是违规求助? 2891780
关于积分的说明 8268752
捐赠科研通 2559811
什么是DOI,文献DOI怎么找? 1388701
科研通“疑难数据库(出版商)”最低求助积分说明 650798
邀请新用户注册赠送积分活动 627775