Alendronate-decorated biodegradable polymeric micelles for potential bone-targeted delivery of vancomycin

胶束 PLGA公司 药物输送 化学 乙二醇 纳米医学 生物物理学 材料科学 毒品携带者 核化学 纳米颗粒 有机化学 纳米技术 水溶液 生物
作者
Yirui Cong,Changyun Quan,Meiqing Liu,Jie Liu,Guohong Huang,Guoquan Tong,Yi Yin,Chao Zhang,Qing Jiang
出处
期刊:Journal of Biomaterials Science-polymer Edition [Informa]
卷期号:26 (11): 629-643 被引量:43
标识
DOI:10.1080/09205063.2015.1053170
摘要

Osteomyelitis is a bone infection disease which is caused by bacteria or other germs, and could cause serious impact on the health and working capacity of the patients. Alendronate (ALN) can chelate strongly with the calcium ion of hydroxyapatite (HA) which is commonly used to treat osteoporosis. Nanomedicine has attracted a lot of attention in that the nano-sized carrier can deliver drug molecules to specific site of interest with the aid of targeting moiety and achieve sustained release, resulting in improved therapeutic effect and reduced side effect. In this study, micelles self-assembled from poly(lactic acid-co-glycolic acid)-block-poly(ethylene glycol)-alendronate (PLGA-PEG-ALN) copolymer were prepared for bone-targeted delivery of vancomycin (Van). The chemical structure of PLGA-PEG-ALN was confirmed by proton nuclear magnetic resonance ((1)H-NMR) spectroscopy. The formation of the nanoparticles was characterized by dynamic light scattering, transmission electronic microscopy as well as the critical micelle concentration measurement. Release profiles from the micelles revealed that the conjugation of ALN to the surface of micelle did not pose adverse effect on the drug-loading capacity and release behaviors. The cytotoxicity of Van-loaded PLGA-PEG-ALN micelles as well as the blank micelles was evaluated via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay toward rat bone marrow stromal cells (rBMSCs) and human embryonic hepatocytes (L02 cells), and results showed that this Van-loaded micelle possesses appropriate cytotoxicity and is safe in the potential treatment of osteomyelitis. The in vitro affinity of PLGA-PEG-ALN micelles to the HA was also confirmed in vitro. The antibacterial effect of Van-loaded PLGA-PEG-ALN micelles was tested against Staphylococcus aureus (SA) which is the main pathogenic bacteria in osteomyelitis, and the results showed that the Van-loaded micelles can effectively inhibit the growth of SA. These results demonstrated that the PLGA-PEG-ALN micelles may be potentially used for the bone targeted delivery of Van.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
weiweiwei发布了新的文献求助10
刚刚
qingniujushi完成签到,获得积分20
刚刚
flow发布了新的文献求助20
1秒前
1秒前
医研丁真完成签到 ,获得积分10
1秒前
2秒前
NIER完成签到,获得积分20
2秒前
2秒前
2秒前
3秒前
学术小白完成签到 ,获得积分10
4秒前
在水一方应助缺粥采纳,获得10
5秒前
5秒前
6秒前
鳄鱼队长发布了新的文献求助50
6秒前
想法高峰发布了新的文献求助10
7秒前
lincool完成签到,获得积分10
7秒前
liaowei0021发布了新的文献求助10
8秒前
爱科研完成签到 ,获得积分10
9秒前
逍遥完成签到 ,获得积分10
9秒前
畅快远山完成签到,获得积分10
9秒前
10秒前
周周完成签到,获得积分10
10秒前
10秒前
11秒前
11秒前
5Hz完成签到,获得积分20
11秒前
SJT完成签到,获得积分10
11秒前
科目三应助林夕采纳,获得30
11秒前
TTKX完成签到,获得积分10
12秒前
12秒前
马佳凯发布了新的文献求助10
13秒前
13秒前
Arhtur完成签到,获得积分10
14秒前
椰水冰凉完成签到,获得积分10
15秒前
15秒前
15秒前
bastien完成签到,获得积分10
15秒前
在水一方应助潇洒诗云采纳,获得10
16秒前
16秒前
高分求助中
Lire en communiste 1000
Ore genesis in the Zambian Copperbelt with particular reference to the northern sector of the Chambishi basin 800
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 700
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 700
Becoming: An Introduction to Jung's Concept of Individuation 600
Die Gottesanbeterin: Mantis religiosa: 656 500
Communist propaganda: a fact book, 1957-1958 500
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3170629
求助须知:如何正确求助?哪些是违规求助? 2821693
关于积分的说明 7936030
捐赠科研通 2482134
什么是DOI,文献DOI怎么找? 1322290
科研通“疑难数据库(出版商)”最低求助积分说明 633607
版权声明 602608