Expression of Polycomb-group genes in human ovarian follicles, oocytes and preimplantation embryos

Mammalian oocytes possess unique properties with respect to their ability to regulate and reprogram chromatin structure and epigenetic information. Proteins containing the conserved chromodomain motif that is common to the Polycomb-group (Pc-G) proteins and the heterochromatin-associated protein HP1, play essential roles in these processes and more specifically, in X-chromosome inactivation in female zygotes and extra-embryonic tissues and in the regulation of genomic imprinting. To characterize the potential role of these proteins in the regulation of epigenetic events during early human development, we utilized a degenerate PCR priming assay to assess the expression of mRNAs of chromodomain proteins in cDNA samples derived from the human female germline and preimplantation embryos. Expression of mRNAs of HP1 genes was observed in ovarian follicles, ( HP1 HSα , HP1 HSβ , HP1 HSγ ), mature oocytes ( HP1 HSα , HP1 HSβ ), cleavage stage preimplantation embryos ( HP1 HSα , HP1 HSβ , HP1 HSγ ) and blastocysts ( HP1 HSα , HP1 HSγ ). Transcripts for three Pc-G genes, which are essential for early mammalian development ( Yin Yang 1 ( YY1 ), Enhancer of Zeste-2 ( EZH2 ) and Embryonic Ectoderm Development ( EED )) and that are essential for the regulation of X-inactivation and certain imprinted genes ( EED ) were revealed by gene-specific-PCR expression analysis of human ovarian follicles, oocytes and preimplantation embryos. YY1 a nd EZH2 transcripts were additionally detected in metaphase II oocytes.