过氧化物酶
化学
谷胱甘肽
生物化学
GPX6型
GPX3型
谷胱甘肽过氧化物酶
酶
标识
DOI:10.1016/s0021-9258(18)70234-2
摘要
phenylhydrazine and hydrogen peroxide were prepared fresh as needed for the various experimental procedures. The hydrogen peroxide concentration was determined by titration with a standardized permanganate solution. Horseradish peroxidase was obtained from Nutritional Biochemicals Corporation. Diethylaminoethyl cellulose1 was prepared from Solka-Floe as described by Peterson and Sober (8). Titration curves of the lyophilized preparation showed the presence of 0.9 m.eq. of ionizing groups per gm. of dry material. Methods-All spectrophotometric measurements were made with a model DU Beckman spectrophotometer. Optical density readings at 270 rnp were used as a routine measure of the protein concentration in eluent fractions. The optical density readings at 270 rnp of a solution of the purified enzyme preparation were correlated with the protein nitrogen content of this same sample by a nitrogen analysis carried out by the Kjeldahl procedure (9). Catalase activity was measured by the method of Feinstein (10).
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