IQGAP3 is essential for cell proliferation and motility during zebrafish embryonic development

生物 细胞生物学 斑马鱼 吗啉 IQGAP1型 成纤维细胞生长因子 成纤维细胞生长因子受体1 支架蛋白 信号转导 受体 遗传学 基因
作者
Xiaolan Fang,Bianhong Zhang,Bernard Thisse,George S. Bloom,Christine Thisse
出处
期刊:Cytoskeleton [Wiley]
卷期号:72 (8): 422-433 被引量:15
标识
DOI:10.1002/cm.21237
摘要

IQGAPs are scaffolding proteins that regulate actin assembly, exocyst function, cell motility, morphogenesis, adhesion and division. Vertebrates express 3 family members: IQGAP1, IQGAP2, and IQGAP3. IQGAP1 is known to stimulate nucleation of branched actin filaments through N‐WASP and the Arp2/3 complex following direct binding to cytoplasmic tails of ligand‐activated growth factor receptors, including EGFR, VEGFR2 and FGFR1. By contrast, little is known about functions of IQGAP2 or IQGAP3. Using in situ hybridization on whole mount zebrafish ( Danio rerio ) embryos, we show that IQGAP1 and IQGAP2 are associated with discrete tissues and organs, while IQGAP3 is mainly expressed in proliferative cells throughout embryonic and larval development. Morpholino knockdowns of IQGAP1 and IQGAP2 have little effect on embryo morphology while loss of function of IQGAP3 affects both cell proliferation and cell motility. IQGAP3 morphant phenotypes are similar to those resulting from overexpression of dominant negative forms of Ras or of Fibroblast Growth Factor Receptor 1 (FGFR1), suggesting that IQGAP3 plays a role in FGFR1‐Ras‐ERK signaling. In support of this hypothesis, dominant negative forms of FGFR1 or Ras could be rescued by co‐injection of zebrafish IQGAP3 mRNA, strongly suggesting that IQGAP3 acts as a downstream regulator of the FGFR1‐Ras signaling pathway. © 2015 Wiley Periodicals, Inc.
最长约 10秒,即可获得该文献文件

科研通智能强力驱动
Strongly Powered by AbleSci AI
更新
大幅提高文件上传限制,最高150M (2024-4-1)

科研通是完全免费的文献互助平台,具备全网最快的应助速度,最高的求助完成率。 对每一个文献求助,科研通都将尽心尽力,给求助人一个满意的交代。
实时播报
1秒前
1秒前
1秒前
不回首发布了新的文献求助10
2秒前
漾漾发布了新的文献求助10
2秒前
昔时旧日发布了新的文献求助10
2秒前
一蓑烟雨任平生给一蓑烟雨任平生的求助进行了留言
4秒前
沐頭完成签到,获得积分10
4秒前
5秒前
zjl123发布了新的文献求助10
5秒前
科研通AI2S应助彘shen采纳,获得10
8秒前
9秒前
9秒前
桐桐应助学术脑袋采纳,获得10
9秒前
香蕉觅云应助jiangnan采纳,获得10
9秒前
科目三应助漾漾采纳,获得10
10秒前
未来可期发布了新的文献求助100
10秒前
小马甲应助研友_nEoEy8采纳,获得10
11秒前
xiaoxiao完成签到,获得积分10
11秒前
科研小虫应助柴胡采纳,获得10
11秒前
慕青应助科研通管家采纳,获得10
12秒前
在水一方应助科研通管家采纳,获得10
12秒前
FashionBoy应助科研通管家采纳,获得10
12秒前
科研通AI2S应助科研通管家采纳,获得10
12秒前
斯文败类应助科研通管家采纳,获得30
12秒前
酷波er应助科研通管家采纳,获得50
12秒前
汉堡包应助科研通管家采纳,获得10
12秒前
13秒前
cctv18应助科研通管家采纳,获得10
13秒前
orixero应助科研通管家采纳,获得10
13秒前
juziyaya应助科研通管家采纳,获得100
13秒前
13秒前
13秒前
Rank发布了新的文献求助30
13秒前
我是一个无趣的人完成签到,获得积分10
13秒前
14秒前
15秒前
酷波er应助月亮馅饼采纳,获得20
15秒前
15秒前
田様应助儒雅的夏山采纳,获得10
15秒前
高分求助中
The late Devonian Standard Conodont Zonation 2000
Nickel superalloy market size, share, growth, trends, and forecast 2023-2030 2000
The Lali Section: An Excellent Reference Section for Upper - Devonian in South China 1500
Smart but Scattered: The Revolutionary Executive Skills Approach to Helping Kids Reach Their Potential (第二版) 1000
Very-high-order BVD Schemes Using β-variable THINC Method 850
Mantiden: Faszinierende Lauerjäger Faszinierende Lauerjäger 800
PraxisRatgeber: Mantiden: Faszinierende Lauerjäger 800
热门求助领域 (近24小时)
化学 医学 生物 材料科学 工程类 有机化学 生物化学 物理 内科学 纳米技术 计算机科学 化学工程 复合材料 基因 遗传学 催化作用 物理化学 免疫学 量子力学 细胞生物学
热门帖子
关注 科研通微信公众号,转发送积分 3251944
求助须知:如何正确求助?哪些是违规求助? 2894827
关于积分的说明 8283422
捐赠科研通 2563461
什么是DOI,文献DOI怎么找? 1391552
科研通“疑难数据库(出版商)”最低求助积分说明 651860
邀请新用户注册赠送积分活动 628894