紫杉醇
拉曼光谱
共焦
共焦显微镜
显微镜
药品
化学
癌细胞
生物物理学
癌症
药理学
病理
生物
医学
光学
细胞生物学
内科学
物理
作者
Hamideh Salehi,L. Derely,Attila-Gergely Végh,Jean‐Cédric Durand,Csilla Gergely,Christian Larroque,M.-A. Fauroux,Frédéric Cuisinier
摘要
Confocal Raman microscopy, a non-invasive, label-free, and high spatial resolution imaging technique is employed to trace the anticancer drug paclitaxel in living Michigan Cancer Foundation-7 (MCF-7) cells. The Raman images were treated by K-mean cluster analysis to detect the drug in cells. Distribution of paclitaxel in cells is verified by calculating the correlation coefficient between the reference spectrum of the drug and the whole Raman image spectra. A time dependent gradual diffusion of paclitaxel all over the cell is observed suggesting a complementary picture of the pharmaceutical action of this drug based on rapid binding of free tubulin to crystallized paclitaxel.
科研通智能强力驱动
Strongly Powered by AbleSci AI