足细胞
雷公藤甲素
波多辛
突触素
尼福林
嘌呤霉素
体外
体内
药理学
化学
医学
细胞生物学
生物
肾
内科学
生物化学
蛋白质生物合成
蛋白尿
细胞凋亡
生物技术
作者
Zheng Cai,Zhaohong Chen,Caihong Zeng,Weisong Qin,LI Leishi,Zhihong Liu
摘要
Extracts of Tripterygium wilfordii Hook F have been used to treat glomerulonephritis for more than 30 years in China with dramatic antiproteinuric effects. Triptolide, a diterpene triepoxide, is one of the major active components of these extracts. To clarify its antiproteinuric effects we induced podocyte injury by puromycin aminonucleoside. Triptolide effectively reduced the proteinuria induced by puromycin in nephrotic rats without reducing the glomerular filtration rate. The antiproteinuric effect was associated with improvement in the foot process effacement, a decrease in the podocyte injury marker desmin as well as the restoration of nephrin and podocin expression and distribution. In cultured mouse podocytes triptolide pretreatment prevented the puromycin-induced disruption of the actin cytoskeleton and microfilament-associated synaptopodin while protecting nephrin and podocin expression. Triptolide suppressed reactive oxygen species generation and p38 mitogen-activated protein kinase activation while restoring RhoA signaling activity. These results show that triptolide ameliorates puromycin aminonucleoside-mediated podocyte injury in vivo and in vitro.
科研通智能强力驱动
Strongly Powered by AbleSci AI