DeepSinse: deep learning based detection of single molecules.

Motivation Imaging single molecules has emerged as a powerful characterization tool in the biological sciences. The detection of these under various noise conditions requires the use of algorithms that are dependent on the end-user inputting several parameters, the choice of which can be challenging and subjective. Results In this work, we propose DeepSinse, an easily-trainable and useable deep neural network that can detect single molecules with little human input and across a wide range of signal-to-noise ratios. We validate the neural network on the detection of single bursts in simulated and experimental data and compare its performance with the best-in-class, domain-specific algorithms. Availability Ground truth ROI simulating code, neural network training, validation code, classification code, ROI picker, GUI for simulating, training and validating DeepSinse as well as pre-trained networks are all released under the MIT License on www.github.com/jdanial/DeepSinse.The dSTORM dataset processing code is released under the MIT License on www.github.com/jdanial/StormProcessor. Supplementary information Supplementary data are available at Bioinformatics online.