丝素
丝绸
傅里叶变换红外光谱
红外线的
表征(材料科学)
傅里叶变换
纳米技术
材料科学
化学
拉曼光谱
化学工程
分析化学(期刊)
复合材料
光学
色谱法
物理
工程类
量子力学
作者
Jiajia Zhong,Xiaojie Zhou,Chao Ye,Wenjie Yu,Yuzhao Tang
出处
期刊:Methods in molecular biology
日期:2021-01-01
卷期号:: 207-219
被引量:4
标识
DOI:10.1007/978-1-0716-1574-4_18
摘要
The secondary structures of silk fibroin (SF) are critical in the determination of the mechanical properties of the animal silks. Different characterization techniques, such as X-ray diffraction, nuclear magnetic resonance, Raman spectroscopy, and Fourier transform infrared (FTIR) technique, have been applied to study the secondary structure of animal silks. Among these techniques, FTIR is most widely used as it is sensitive to all secondary structures of proteins. Especially with the development of FTIR imaging, it is now possible to image the secondary structures of proteins at the micrometer scale, so as to understand the spatial distribution of proteins and the interaction of proteins with other materials at specific locations of interest. In this chapter, we present the methods and protocols of FTIR imaging to silk protein-based materials. We primarily introduce how to set up the instruments and accessories, as well as how to choose the appropriate imaging methods and sample preparation methods according to sample morphologies. The critical protocols for data analysis are also introduced in the last section.
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